毒素T3DB ID

T3D3773

目标BioDB ID

BE0004810

目标名称

RNA

目标UniProt ID

作用机理

alpha-Sarcin is a highly specific ribotoxin that inhibits protein synthesis by cleaving a single phosphodiester bond in the sarcin/ricin loop (SRL) of the 23S鈭?8S rRNA of eukaryotic ribosomes. The sequence around this cleavage site is a binding site for elongation factors, and is conserved in all cytoplasmic ribosomes. In order to cleave this site, alpha-sarcin binds to the rRNA by forming an electrostatic complex using specific residues on its active site face. This cleavage prevents the binding of elongation factors to the ribosome, halts protein synthesis, and eventually leads to apoptotic cell death. (A3082, A3084, A3086, A3087)

参考文献

A3082 - Korennykh AV, Plantinga MJ, Correll CC, Piccirilli JA: Linkage between substrate recognition and catalysis during cleavage of sarcin/ricin loop RNA by restrictocin. Biochemistry. 2007 Nov 6;46(44):12744-56. Epub 2007 Oct 12. (17929942) A3084 - Martinez-Ruiz A, Kao R, Davies J, Martinez del Pozo A: Ribotoxins are a more widespread group of proteins within the filamentous fungi than previously believed. Toxicon. 1999 Nov;37(11):1549-63. (10482390) A3086 - Sacco G, Drickamer K, Wool IG: The primary structure of the cytotoxin alpha-sarcin. J Biol Chem. 1983 May 10;258(9):5811-8. (6343394) A3087 - Correll CC, Yang X, Gerczei T, Beneken J, Plantinga MJ: RNA recognition and base flipping by the toxin sarcin. J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):93-6. Epub 2003 Nov 28. (14646144)

创建于

2010-05-26 16:24:12 UTC

更新于

2014-09-04 16:18:49 UTC