毒素T3DB ID

T3D3771

目标BioDB ID

BE0004810

目标名称

RNA

目标UniProt ID

作用机理

Restrictocin is a highly specific ribotoxin that inhibits protein synthesis by cleaving a single phosphodiester bond in the sarcin/ricin loop (SRL) of the 23S鈭?8S rRNA of eukaryotic ribosomes. The sequence around this cleavage site is a binding site for elongation factors, and is conserved in all cytoplasmic ribosomes. In order to cleave this site, restrictocin binds to the rRNA by forming an electrostatic complex using specific residues on its active site face. This cleavage prevents the binding of elongation factors to the ribosome, halts protein synthesis, and eventually leads to apoptotic cell death. (A3081, A3082, A3083)

参考文献

A3081 - Yang X, Moffat K: Insights into specificity of cleavage and mechanism of cell entry from the crystal structure of the highly specific Aspergillus ribotoxin, restrictocin. Structure. 1996 Jul 15;4(7):837-52. (8805570) A3082 - Korennykh AV, Plantinga MJ, Correll CC, Piccirilli JA: Linkage between substrate recognition and catalysis during cleavage of sarcin/ricin loop RNA by restrictocin. Biochemistry. 2007 Nov 6;46(44):12744-56. Epub 2007 Oct 12. (17929942) A3083 - Plantinga MJ, Korennykh AV, Piccirilli JA, Correll CC: Electrostatic interactions guide the active site face of a structure-specific ribonuclease to its RNA substrate. Biochemistry. 2008 Aug 26;47(34):8912-8. doi: 10.1021/bi800592g. Epub 2008 Aug 2. (18672906)

创建于

2010-05-26 15:45:33 UTC

更新于

2014-09-04 16:18:49 UTC