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咖啡因-caffeine
记事
A2350T was inserted into rabbit RYR1 and compared to wild type in transfected HEK-293 cells. Expressed protein was isolated as crude membrane fraction, which was used to perform [3H]ryanodine binding experiments, which suggest that suggests that the A2350T mutant channel opens at lower and closes at higher concentrations of calcium. Caffeine increases both the wild-type and mutant RyR1 protein bind more [3H]ryanodine. However, the A2350T mutant was more affected by caffeine compared to the wild type.
专家论述
Allele A is associated with increased sensitivity to caffeine in HEK-293 cells as compared to allele G.