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“美苯妥英”、“奥美拉唑”-"mephenytoin","omeprazole"
药物名称(英)
"mephenytoin","omeprazole"
记事
Healthy volunteers (113 Vietnamese and 94 Korean) received a single 20 mg dose of omeprazole after overnight fasting. Blood samples were taken 3 hr after administration of omeprazole. Samples from volunteers with 1) 1/*2 or *1/*3 genotypes and 2) a metabolic ratio (MR) of omeprazole/ 5-hydroxyomeprazole that was higher than the average MR for *2/*2 or *3/*3 underwent direct DNA sequencing of CYP2C19 to search for novel variants responsible for the high MR. The corresponding variants were recreated by site-directed mutagenesis of CYP2C19 cDNA which was integrated into plasmids and transfected into DH5 alpha "E.coli" cells. CYP2C19 protein variants were eluted and tested for their effect on enzymatic activity. *Note: the protein change that corresponds to rs55948420 A is V394M which has no associated *allele.
专家论述
Allele A is not associated with catalytic activity of CYP2C19 when exposed to mephenytoin and omeprazole as compared to allele G.