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变量_单倍型
CYP2C19*1, CYP2C19*18
记事
A mutation for CYP2C19*1C (991A > G) was introduced using pcDNA3.1/CYP2C19*1A as a template, resulting in pcDNA3.1/CYP2C19*1C (*1B and *1C is used interchangeably in the article). Mutations for CYP2C19*18 (991A > G and 986G>A) was successively introduced using pcDNA3.1/CYP2C19*1C as a template. All CYP2C19 plasmids were sequenced to confirm successful mutagenesis. The cDNAs of CYP2C19*1C, CYP2C19*18 were subsequently subcloned into the pGYR1 yeast expression vector and heterologously expressed in yeast cells and proteins were purified from yeast microsomes. Proteins levels were assayed by reduced CO difference spectra and immunoblot.
专家论述
CYP2C19 *18 is associated with increased expression of CYP2C19 as compared to CYP2C19 *1.